Resources

Software

Most software code and scripts developed by the Huang Lab are available at Bo Huang Lab GitHub⁵.
Compressed sensing for STORM:
- Faster STORM using compressed sensing⁶.
- Download the Matlab code and sample data: Zip file: compressed sensing for storm (227 KB)⁷
Drift correction for STORM:
- Localization events-based sample drift correction for localization microscopy with redundant cross-correlation algorithm⁸.
- Download the Matlab code: Zip file: drift correction for storm (6 KB)⁹

Plasmids

We have deposited plasmids from our lab at Bo Huang Lab AddGene¹⁰.

Also see our CRISPR imaging resource page¹¹.

AddGene is unable to deliver dCas9-GFP in the Tet-On 3G system due to licensing issues. Therefore, we have deposited a template plasmid¹² for cloning into inducible expressions that can be obtained from commercial or academic channels.

Certain plasmids are not deliverable through AddGene due to licensing issues. Contact us¹³ and we are happy to send these plasmids directly.

Cell lines

Although we have generated a large number of gene-edited cell lines, we are not maintaining all of them because of the extensive resources required to do so. Have a specific need? Contact us¹³ and we will try our best to help.

Links

https://www.ucsf.edu/

https://pharmacy.ucsf.edu

https://www.ucsfhealth.org/

https://huanglab.ucsf.edu/

https://github.com/BoHuanglab

https://www.nature.com/articles/nmeth.1978

https://huanglab.ucsf.edu/sites/pharm.ucsf.edu/files/huang/media-browser/compressed-sensing-for-storm.zip

https://www.researchgate.net/publication/263549486_Localization_events-based_sample_drift_correction_for_localization_microscopy_with_redundant_cross-correlation_algorithm

https://huanglab.ucsf.edu/sites/pharm.ucsf.edu/files/huang/media-browser/drift-correction-for-storm.zip

https://www.addgene.org/Bo_Huang/

http://huanglab.ucsf.edu/CRISPRimaging.html

https://www.addgene.org/51023/

https://huanglab.ucsf.edu/contact