Contact info
UCSF Byers Hall Box 2532
1700 4th St Floor 3 Rm 303A
San Francisco CA 94143
FedEx only: use zip 94158
tel: 415-476-1866
fax: 415-514-1028
Employment
| 2017- | Investigator, Chan Zuckerberg Biohub |
| 2017- | Professor, Department of Pharmaceutical Chemistry and Department of Biochemistry and Biophysics, UCSF |
| 2014-2017 | Associate Professor, Department of Pharmaceutical Chemistry and Department of Biochemistry and Biophysics, UCSF |
| 2009-2014 | Assistant Professor, Department of Pharmaceutical Chemistry and Department of Biochemistry and Biophysics, UCSF |
Academic training
| 2006-2009 | Harvard University | Postdoctoral Fellow | Mentor: Xiaowei Zhuang |
| 2001-2006 | Stanford University | PhD in Chemistry | Advisor: Richard N. Zare, Thesis: Chemical analysis of single cells |
| 1997-2001 | Peking University, China | BS in Chemistry, summa cum laude |
Honors received
| 2017 | UCSF Byers Award in Basic Science |
| 2017-2027 | Chan Zuckerberg Biohub Investigator |
| 2016-2018 | Finalist, Blavatnik National Awards for Young Scientists |
| 2016 | ASCB Early Career Life Scientist Award |
| 2014 | W.M. Keck Foundation Medical Research Grant |
| 2011 | NIH Director’s New Innovator Award |
| 2010 | Packard Fellowship for Science and Engineering |
| 2010 | Searle Scholars |
| 2007 | GE & Science Young Life Scientist Prize, North American Regional Winner |
| 2006 | Annual Reviews Prize in Physical Chemistry, Stanford University |
| 2001-2004 | Stanford Graduate Fellowship |
| 1996 | Rado Young Genius Prize in China, First Place |
Selected publications
- A. Berrocal*, J.E. Sandoval*, N. Khetan*, A. Ma, T. Wang, C. Moore, G.J. Narlikar, H. Li, D.G. Fujimori#, B. Huang#. "Preferential formation of NUP98-KDM5A condensates at specific H3K4me3-rich loci drives leukemogenic gene expression". eLife 15, PR111922 (2026).
- Y. Chen*, K. Yserentant*, K. Hong*, Y. Kuang, R.S. Picardo, A. Bhowmick, A. Charles-Orszag, S.J. Lord, L. Lu, K. Hou, S.I. Mann, S. Bhattacharya, M. Horst, J.B. Grimm, L.D. Lavis, R.D. Mullins, W.F. DeGrado#, B. Huang#. "De novo designed bright, hyperstable rhodamine binders for fluorescence microscopy". Cell (2026).
- I.E. Ivanov,*,# E. Hirata-Miyasaki,* T. Chandler,* R. Cheloor-Kovilakam,* Z. Liu, S. Pradeep, C. Liu, M. Bhave, S. Khadka, C. Arias, M.D. Leonetti, B. Huang,# S.B. Mehta#, "Mantis: high-throughput 4D imaging and analysis of the molecular and physical architecture of cells," PNAS Nexus, pgae323 (2024).
- E. Khwaja, Y.S. Song, A. Agarunov, B. Huang, "CELL-E 2: Translating Proteins to Pictures and Back with a Bidirectional Text-to-Image Transformer," In: 37th Neural Information Processing Systems NeurIPS (2023).
- J. Zhao*, S. Makhija*, C. Zhou, H. Zhang, Y.Q. Wang, M. Muralidharan, B. Huang#, Y. Cheng#, "Structural insights into the human PA28–20S proteasome enabled by efficient tagging and purification of endogenous proteins," Proc. Nat. Acad. Sci. U.S.A., 119, e2207200119 (2022).
- Y. Wang, H. Pinkard, E. Khwaja, S. Zhou, L. Waller, B. Huang, “Image denoising for fluorescence microscopy by supervised to self-supervised transfer learning,” Opt. Express 29, 41303-41312 (2021).
- X. Shi*, Q. Li*, Z. Dai, A.A. Tran, S. Feng, A.D. Ramirez, Z. Lin, X. Wang, T.T. Chow, J. CHen, D. Kumar, A.R. McColloch, J.F. Reiter, E.J. Huang, I.B. Seiple#, B. Huang#, “Label-retention expansion microscopy,” J. Cell Biol., 220, e202105067 (2021).
- A. Tulpule*, J. Guan*, D.S. Neel*, H.R. Allegakoen, Y.P. Lin, D. Brown, Y. Chou, A. Heslin, N. Chatterjee, S. Perati, S. Menon, T.A. Nguyen, J. Debnath, A.D. Ramirez, X. Shi, B. Yang, S. Feng, S. Makhija, B. Huang#, T.G. Bivona#, “Kinase-mediated RAS signaling via membraneless cytoplasmic protein,” Cell 184, 2649-2664.e18 (2021).
- S. Makhija, D. Brown, R.M. Rudlaff, J. Doh, S. Bourke, Y. Wang, S. Zhou, R. Cheloor-Kovilakam, B. Huang, “Versatile labeling and detection of endogenous proteins using tag-assisted split enzyme complementation,” ACS Chem. Biol. 16, 671-681 (2021).
- B. Yang, X. Chen, Y. Wang, S. Feng, V. Pessino, N. Stuurman, N.H. Cho, K.W. Cheng, S.J. Lord, L. Xu, D. Xie, R.D. Mullins, M.D. Leonetti, B. Huang, “Epi-illumination SPIM for volumetric imaging with high spatial-temporal resolution,” Nat. Methods 16, 501-504 (2019).
- B. Chen, W. Zou, H. Xu, Y. Liang, B. Huang, “Efficient labeling and imaging of protein-coding genes in living cells using CRISPR-Tag,” Nat. Commun. 9, 5065 (2018).
- J. Schnitzbauer, Y. Wang, M. Bakalar, B. Chen, T. Nuwal, S. Zhao, B. Huang, “Correlation analysis framework for localization-based superresolution microscopy,” Proc. Nat. Acad. Sci. USA 115, 3219-3224 (2018).
- X. Shi, G. Garcia, J.C. Van De Weghe, R. McGorty, G.J. Pazour, D. Doherty, B. Huang*, J.F. Reiter*, “Super-resolution microscopy reveals that disruption of ciliary transition-zone architecture causes Joubert syndrome,” Nat. Cell Biol. 19, 1178-1188 (2017).
- R. McGorty, D. Xie, B. Huang, “High-NA open-top selective-plane illumination microscopy for biological imaging,” Opt. Express, 25, 17798-17810 (2017).
- S. Feng, S. Sekine, V. Pessino, H. Li, M.D. Leonetti, B. Huang, “Improved split fluorescent proteins for endogenous protein labeling,” Nat. Commun., 8, 370 (2017).
- J. Guan, H. Liu, X. Shi, S. Feng, B. Huang, “Tracking multiple genomic elements using correlative CRISPR imaging and sequential DNA FISH,” Biophys. J., 112, 1077-1084 (2017).
- M.D. Leonetti, S. Sekine, D. Kamiyama, J.S. Weissman, B. Huang, “A scalable strategy for high-throughput GFP tagging of endogenous human proteins,” Proc. Nat. Acad. Sci. USA, 113, E3501-E3508 (2016).
- D. Kamiyama, S. Sekine, B. Barsi-Rhyne, J. Hu, B. Chen, L.A. Gilbert, H. Ishikawa, M.D. Leonetti, W.F. Marshall, J.S. Weissman, B. Huang, “Versatile protein tagging in cells with split fluorescent protein,” Nat. Commun., 7, 11046 (2016).
- B. Chen, J. Hu, R. Almeida, H. Liu, S. Balakrishnan, C. Covill-Cooke, W.A. Lim, B. Huang, “Expanding the CRISPR imaging toolset with Staphylococcus aureus Cas9 for simultaneous imaging of multiple genomic loci,” Nucleic Acids Res. 44, e75 (2016).
- D. Kamiyama, R. McGorty, R. Kamiyama, M.D. Kim, A. Chiba, B. Huang, “Specification of dendritogenesis site in Drosophila aCC motoneuron by membrane-enrichment of Pak1 through Dscam1,” Dev. Cell, 35, 93-106 (2015).
- B. Chen, L.A. Gilbert, B.A. Cimini, J. Schnitzbauer, W. Zhang, G.W. Li, J. Park. E.H. Blackburn, J.S. Weissman, L.S. Qi, B. Huang, “Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system,” Cell, 155, 1479-1491 (2013).
- E.M. Puchner, J.M. Walter, R. Kasper, B. Huang*, W.A. Lim*, “Counting molecules in single organelles with superresolution microscopy allows tracking of the endosome maturation trajectory,” Proc. Nat. Acad. Soc. USA, 110, 16015-16020 (2013).
- L. Zhu, W. Zhang, D. Elnatan, B. Huang, “Faster STORM with compressed sensing,” Nat. Methods, 9, 721-723 (2012).
Professional activities
- Instructor, 2010 Bangalore Microscopy Course
- Instructor, 2011, 2013, 2016, 2017 Cold Spring Harbor Laboratory Course in Quantitative Imaging
- Co-organizer, 2012-2014 QB3-UCSF Course on Biological Microscopy
- Member, Biophysical Society (2002-Date)
- Member, American Chemistry Society (2005-Date)
- Member, American Society of Cell Biology (2008-Date)
- Journal Reviewer: Cell, Nature, Science, Nature Methods, Nature Biotechnology, Nature Cell Biology, Nature Communications, PNAS, eLife, Developmental Cell, Journal of American Chemical Society, Structure, Nano Letter, Nanoscale, Journal of Molecular Biology, Biophysical Journal, Optica, Optics Letter, Optics Express, Biomedical Optics Express, Microscopy Research and Technique, PLoS One, Scientific Report, etc.