Bo Huang          Curriculum vitae         Short Version

1700 4th St, MC 2532
Byers Hall 303A
San Francisco, CA 94143

Tel: (415) 476-1866
Fax: (415) 514-1028


Associate Professor, Department of Pharmaceutical Chemistry and Department of Biochemistry and Biophysics, UCSF 2014-
Assistant Professor, Department of Pharmaceutical Chemistry and Department of Biochemistry and Biophysics, UCSF 2009-2014

Academic Training

Harvard University
    Postdoctoral Fellow                     Mentor: Xiaowei Zhuang
Stanford University
    Ph.D. in Chemistry                      Advisor: Richard N. Zare
    Thesis: Chemical analysis of single cells
Peking University, China
    B.S. in Chemistry summa cum laude

Honors Received

♦  Chan Zuckerberg Biohub Investigator 2017-2022
♦  ASCB Early Career Life Scientist Award 2016
♦  NIH Director's New Innovator Award 2011
♦  Packard Fellowship for Science and Engineering 2010
♦  Searle Scholars 2010
♦  GE & Science Young Life Scientist Prize
    North American Regional Winner
♦  Annual Reviews Prize in Physical Chemistry,
    Stanford University
♦  Stanford Graduate Fellowship 2001-2004
♦  Guang Cai Scholarship, Peking University 1997-2000
♦  Honorable Mention in Interdisciplinary Contest in Modeling
    The Consortium for Mathematics and its Applications, USA
♦  Bronze Medal in the National Mathematics Olympiad, China 1997
♦  Meritorious in Mathematical Contest in Modeling
    The Consortium for Mathematics and its Applications, USA
♦  Rado Young Genius Prize in China, First Place 1996


  • 65. R. Irannejad, V. Pessino, D. Mika, B. Huang, P.B. Wedegaertner, M. Conti, M. von Zastrow, "Functional selectivity of GPCR-directed drug action through location bias", Nat. Chem. Biol. in press (2017).
  • 64. B. van Lengerich, E. M. Puchner, C. Angew, B. Huang, N. Jura, "EGF and NRG induce phosphorylation of HER3/ERBB3 by EGFR using distinct oligomeric mechanisms", Proc. Nat. Acad. Sci. USA 114, E2836-3845 (2017).
  • 63. J. Guan, H. Liu, X. Shi, S. Feng, B. Huang, "Tracking multiple genomic elements using correlative CRISPR imaging and sequential DNA FISH", Biophys. J., 112, 1077-1084 (2017).
  • 62. W.Q. Ong, Y.R. Citron, S. Sekine, B. Huang, "Live cell imaging of endogenous mRNA using RNA-based fluorescence "turn-on" probe", ACS Chem. Biol., 12, 200-205 (2017).
  • 61. B. Huang, "A microscopic view of the cell", Mol. Biol. Cell, 27, 3183-3184 (2016).
  • 60. L. Zhu, B. Huang, "STORM using compressed sensing", in Adrian Stern, editor: Optical Compressive Sensing, CRC Press, pp. 225-238 (2016).
  • 59. H. Liu, S. Chen, K. Huang, J. Jim, H. Mo, R. Iovine, J. Gendre, P. Pascal, Q. Li, Z. Dong, M. Arkin, S. Guo, B. Huang, "A high-content larval zebrafish brain imaging method for small molecule drug discovery", PLoS One, 11, e0164645 (2016).
  • 58. Y. Bai, H. Liu, B. Huang, M. Wagle, S. Guo, "Identification of environmental stressors and validation of light preference as a measure of anxiety in larval zebrafish", BMC Neurosci., 17, 63 (2016).
  • 57. M.D. Leonetti, S. Sekine, D. Kamiyama, J.S. Weissman, B. Huang, "A scalable strategy for high-throughput GFP tagging of endogenous human proteins", Proc. Nat. Acad. Sci. USA, 113, E3501-E3508 (2016).
  • 56. B. Chen, J. Guan, B. Huang, "Imaging specific genomic DNA in living cells", Ann. Rev. Biophys., 45, 1-23 (2016).
  • 55. R. McGorty, B. Huang, "Selective-plane illumination microscopy for high-content volumetric biological imaging ", Proc. SPIE, 9720, 97200P, doi:10.1117/12.2212889 (2016).
  • 54. D. Kamiyama, S. Sekine, B. Barsi-Rhyne, J. Hu, B. Chen, L.A. Gilbert, H. Ishikawa, M.D. Leonetti, W.F. Marshall, J.S. Weissman, B. Huang, "Versatile protein tagging in cells with split fluorescent protein", Nat. Commun., 7, 11046 (2016).
  • 53. P. Bieling, T.D. Li, J. Weichsel, R. McGorty, P. Jreij, B. Huang, D.A. Fletcher, R.D. Mullins, "Force feedback controls motor activity and mechanical properties of self-assembling branched actin networks", Cell, 164, 115-127 (2016).
  • 52. B. Chen, J. Hu, R. Almeida, H. Liu, S. Balakrishnan, C. Covill-Cooke, W.A. Lim, B. Huang, "Expanding the CRISPR imaging toolset with Staphylococcus aureus Cas9 for simultaneous imaging of multiple genomic loci", Nucleic Acids Res., 44, e75 (2016).
  • 51. D. Kamiyama, R. McGorty, R. Kamiyama, M.D. Kim, A. Chiba, B. Huang, "Specification of dendritogenesis site in Drosophila aCC motoneuron by membrane-enrichment of Pak1 through Dscam1", Dev. Cell, 35, 93-106 (2015).
  • 50. J.G. Patton, J.L. Franklin, A.M. Weaver, K. Vickers, B. Zhang, R.J. Coffey, K.M. Ansel, R. Blelloch, A. Goga, B. Huang, N. L'Etoille, R. Raffai, C.P. Lai, A.M. Krichevsky, B. Mateescu, V.J. Greiner, C. Hunter, O. Voinnet, M.T. McManus, "Biogenesis, delivery and function of extracellular RNA", J. Extracellular Vesicles, 4, 27494 (2015).
  • 49. W.Q. Ong, Y.R. Citron, J. Schnitzbauer, D. Kamiyama, B. Huang, "Heavy water: a simple solution to increasing brightness of fluorescence protein in super-resolution imaging", Chem. Commun.,  51, 13451 - 13453 (2015).
  • 48. R. McGorty, H. Liu, D. Kamiyama. Z. Dong, S. Guo, B. Huang, "Open-top selective plane illumination microscope for conventionally mounted specimens", Opt. Express, 23, 16142-16153 (2015).
  • 47. B. Chen, B. Huang, "Imaging genomic elements in living cells using CRISPR/Cas9". In J.A. Doudna, E.J. Sontheimer, editors: The use of CRISPR/cas9, ZFNs, TALENs in generating site specific genome alterations, Vol 546, MIE, UK: Academic Press, pp. 337-354 (2014).
  • 46. E. Puchner, B. Huang, H. Gaub, "Single molecule techniques - applications in biology", FEBS Lett., 588, 3519 (2014).
  • 45. Y. Wang, J. Schnitzbauer, Z. Hu, X. Li, Y. Cheng, Z.L. Huang, B. Huang, "Localization events-based sample drift correction for localization microscopy with redundant cross-correlation algorithm", Opt. Express, 22, 15982-15991 (2014).
  • 44. S. Sun, C. Zhou, S. Chen, J. Liu, J. Yu, J. Chilek, L. Zhao, M. Y, R. Vinluan, B. Huang, J. Zheng, "Surface-chemistry effect on cellular response of luminescent plasmonic silver nanoparticles", Bioconjugate Chem., 25, 453-458 (2014).
  • 43. R. McGorty, J. Schnitzbauer, W. Zhang, B. Huang, “Correction of depth-dependent aberrations in 3D single molecule localization and super-resolution microscopy”, Opt. Lett., 39, 275-278 (2014).
  • 42. B. Chen, L.A. Gilbert, B.A. Cimini, J. Schnitzbauer, W. Zhang, G.W. Li, J. Park. E.H. Blackburn, J.S. Weissman, L.S. Qi, B. Huang, “Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system”, Cell, 155, 1479-1491 (2013).
  • 41. V. Mennella, D.A. Agard, B. Huang, L. Pelletier, “Amorphous no more: subdiffraction view of the pericentriolar material architecture”, Trends Cell Biol. 24, 188-197. (2014).
  • 40. E.M. Puchner, J.M. Walter, R. Kasper, B. Huang, W.A. Lim, “Counting molecules in single organelles with superresolution microscopy allows tracking of the endosome maturation trajectory”, Proc. Nat. Acad. Soc. USA, 110, 16015-16020 (2013).
  • 39. J. Schnitzbauer, R. McGorty, B. Huang, “4Pi fluorescence detection and 3D particle localization with a single objective”, Opt. Express, 21, 19701-19708 (2013).
  • 38. F. Huang, X. Wang, E.M. Ostertag, T. Nuwal, B. Huang, Y.N. Jan, A.I. Basbaum and L.Y. Jan, “TMEM16C facilitates Na(+)-activated K(+) currents in rat sensory neurons and regulates pain processing”, Nat. Neurosci., 16, 1284-1290 (2013).
  • 37. R. McGorty, D. Kamiyama, B. Huang, “Active microscope stabilization in three dimensions using image correlation”, Optical Nanoscopy, 2, 3 (2013).
  • 36. R. Irannejad, J.C. Tomshine, J.R. Tomshine, M. Chevalier, J. Steyaert, S.G.F. Rasmussen, H. El-Samad, B. Huang, M. von Zastrow, “Conformational biosensors reveal adrenoceptor signaling from endosomes”, Nature, 459, 534-538 (2013).
  • 35. A.S. Stender, K. Marchuk, C. Liu, S. Sander, M.W. Meyer, E.A. Smith, B. Neupane, G. Wang, J. Li, J.X. Cheng, B. Huang, N. Fang, "Single cell optical imaging and spectroscopy", Chem. Rev., 113, 2469-2527 (2013).
  • 34. D. Kamiyama B. Huang, “Development in the STORM”, Developmental Cell, 23, 1103 (2012)
  • 33. V. Mennella, B. Keszthelyi, K.L. McDonald, B.  Chhun, F. Kan, G.C. Rogers, B. Huang, D.A. Agard, “Subdiffraction-resolution fluorescence microscopy reveals a domain of the centrosome critical for pericentriolar material organization”, Nat. Cell Biol., 14, 1159-1168 (2012).
  • 32. S.H. Shim, C. Xia, G. Zhong, H.P. Babcock, J.C. Vaughan, B. Huang, X. Wang, C. Xu, G.Q. Bi, X. Zhuang, “Super-resolution fluorescence imaging of organelles in live cells with photoswitchable membrane probes”, Proc. Nat. Acad. Sci. USA, 109, 13978-3983 (2012).
  • 31. L. Zhu, W. Zhang, D. Elnatan, B. Huang, “Faster STORM with compressed sensing”, Nat. Methods, 9, 721-723 (2012).
  • 30. R. Kasper, B. Huang, “SnapShot: Light Microscopy”, Cell, 147, 1198 (2011).
  • 29. G.M.J. Beaudoin, III, G.M. Schofield, T. Nuwal, K. Zang, E.M. Ullian, B. Huang, L.F. Reichardt, “Afadin, a Ras/Rap effector that controls cadherin function, promotes spine and excitatory synapse density in the hippocampus”, J Neurosci., 32, 99-110 (2012).
  • 28. B. Huang, “An in-depth view”, Nat Methods, 8, 304-305 (2011).
  • 27. B. Huang, H. Babcock, X. Zhuang, “Breaking the diffraction barrier: super-resolution imaging of cells”, Cell, 143, 1047-1058 (2010).
  • 26. A. Dani, B. Huang, J. Bergan, C. Dulac, X. Zhuang, “Superresolution imaging of chemical synapses in the brain”, Neuron, 68, 843-856 (2010).
  • 25. A. Dani, B. Huang, “New resolving power for light microscopy: applications to neurobiology”, Curr. Opin. Neurobiol., 20, 648-652 (2010).
  • 24. M. Wu, B. Huang, M. Graham, A. Raimondi, J.E. Heuser, X. Zhuang, P. De Camilli, “Coupling between clathrin-dependent budding and F-BAR-dependent membrane tubulation in a cell-free system of endocytosis”, Nat. Cell. Biol., 12, 902-908 (2010).
  • 23. B. Huang, “Super resolution optical microscopy: multiple choices”, Curr. Opin. Chem. Biol., 14, 10-14 (2010).
  • 22. B. Huang, M. Bates, X. Zhuang, “Super resolution fluorescence microscopy”, Ann. Rev. Biochem., 78, 993-1016 (2009).
  • 21. M. Bates, B. Huang, X. Zhuang, “Super-resolution imaging by nanoscale localization of photo-switching fluorescent probes”, Curr. Opin. Chem. Biol., 12, 505-514 (2008).
  • 20. B. Huang, S. A. Jones, B. Brandenburg, X. Zhuang, “Whole cell 3D STORM reveals interactions between cellular structures with nanometer-scale resolution”, Nat. Methods, 5, 1047-1052 (2008).
  • 19. B. Huang, W. Wang, M. Bates, X. Zhuang, “Three-dimension super-resolution imaging by stochastic optical reconstruction microscopy”, Science, 319, 810-813 (2008).
  • 18. M. Bates, B. Huang, G. T. Dempsey, X. Zhuang, “Multicolor super-resolution imaging with photo-switchable fluorescent probes”, Science, 317, 1749-1753 (2007).
  • 17. B. Huang, H. Wu, D. Bhaya, A. R. Grossman, S. Granier, B. K. Kobilka, R. N. Zare, “Counting low-copy-number proteins in a single cell”, Science, 315, 81-84 (2007).
  • 16. S. Kim, B. Huang, H. Wu, R. N. Zare, “Microfluidic separation and capture of analytes for single-molecule spectroscopy”, Lab Chip, 7, 1663-1665 (2007).
  • 15. B. Huang, S. Kim, R. N. Zare, “Use of a mixture of n-dodecyl-β-D-maltoside and sodium dodecyl sulfate in poly (dimethylsiloxane) microchips to suppress adhesion and promote separation of proteins”, Anal. Chem., 79, 9145-9149 (2007).
  • 14. M. R. Whorton, M. P. Bokoch, S. G. F. Rasmussen, A. Kuszak, B. Huang, R. N. Zare, B. K. Kobilka, R. K. Sunahara, “A monomeric G protein-coupled receptor isolated in a high-density lipoprotein particle efficiently activates its G protein”, Proc. Nat. Acad. Sci. U.S.A., 104, 7682-7687 (2007).
  • 13. B. Huang, F. Yu, R. N. Zare, “Surface plasmon resonance imaging using a high numerical aperture microscope objective”, Anal. Chem., 79, 2979-2983 (2007).
  • 12. H. Wu, B. Huang, R. N. Zare, “Generation of complex, static solution gradients in microfluidic channels”, J. Am. Chem. Soc., 128, 4194-4195 (2006).
  • 11. Y. Luo, B. Huang, H. Wu, R. N. Zare, “Controlling electroosmotic flow in polydimethylsiloxane (PDMS) separation channels by means of prepolymer additives”, Anal. Chem., 78, 4588-4592 (2006).
  • 10. B. Huang, H. Wu, S. Kim, B. K. Kobilka, R. N. Zare, “Phospholipid biotinylation of polydimethylsiloxane (PDMS) for protein immobilization”, Lab Chip, 6, 369-373 (2006).
  • 9. H. Wu, B. Huang, R. N. Zare, “Construction of microfluidic chips using polydimethylsiloxane for adhesive bonding”, Lab Chip, 5, 1393-1398 (2005).
  • 8. B. Huang, H. Wu, S. Kim, R. N. Zare, “Coating of poly(dimethylsiloxane) with n-dodecyl-beta-D-maltoside to minimize nonspecific protein adsorption”, Lab Chip, 5, 1005-1007 (2005).
  • 7. T. D. Perroud, B. Huang, R. N. Zare, “Effect of bin time on the photon counting histogram for one-photon excitation”, ChemPhysChem, 6, 905-912 (2005).
  • 6. B. Huang, T. D. Perroud, R. N. Zare, “Photon counting histogram: one-photon excitation”, ChemPhysChem, 5, 1523-1531 (2004).
  • 5. T. D. Perroud, B. Huang, M. I. Wallace, R. N. Zare, “Photon counting histogram for one-photon excitation”, ChemPhysChem, 4, 1121-1123 (2003).
  • 4. R. J. Whelan, T. Wohland, L. Neumann, B. Huang, B. K Kobilka, R. N Zare, “Analysis of bimolecular interactions using a miniaturized surface plasmon resonance sensor”, Anal. Chem., 74, 4570-4576 (2002).
  • 3. L. Ying, X. Huang, B. Huang, J. Xie, J. Zhao, X. Zhao, “Fluorescence emission and absorption spectra of single Anabaena sp strain PCC7120 cells”, Photochem. Photobio., 76, 310-313 (2002).
  • 2. L. Ying, X. Huang, B. Huang, J. Zhao, X. Zhao, “Spectra of single cyanobacterial cells”, Acta Physico-Chimica Sinica, 18, 673-675 (2002).
  • 1. B. Huang, Y. Yang, “Treatment of experimental data of surface adsorption in solution by computer linear fitting method”, University Chemistry, 17, 51 (2002).

Invited Presentations

  • Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany (2016)
  • Department of Biomedical Engineering, University of California, Irvine, CA (2016)
  • RIKEN QBiC Symposium, Japan (2016)
  • ICREA International Symposium: BioNanoVision of cellular architecture, Barcelona, Spain (2016)
  • Department of Biochemistry, University of Wisconsin-Madison, WI (2016)
  • Department of Molecular Physiology and Biophysics, Vanderbilt University, TN (2016)
  • 251st American Chemical Society National Meeting, San Diego, CA (2016)
  • Department of Physiology and Biophysics, Weill Cornell College of Medicine, NY (2016)
  • Waters Symposium, Pittsburg Conference on Analytical Chemistry and Applied Spectroscopy, Atlanta, GA (2016)
  • ShanghaiTech Bioforum 2015, Shanghai, China (2015)
  • INSERM workshop: New trends in super-resolution optical microscopy, Bordeaux, Francs (2015)
  • Cold Spring Harbor Laboratory Symposium on Genome Engineering, NY (2015)
  • The 45th World Chemistry Congress and the 48th General Assembly Meeting of the International Union of Pure and Applied Chemistry (IUPAC-2015), Busan, Korea (2015)
  • The 29th Annual Symposium of the Protein Society, Barcelona, Spain (2015)
  • Microscopical Society of Canada 42nd Annual Meeting, Canada (2015)
  • Frontiers of Life Sciences Symposium, School of Life Sciences, Peking University, China (2015)
  • Department of Electric and Computer Engineering, Lehigh University, PA (2015)
  • Bioengineering Lecture Series, California Institute of Technology, CA (2015)
  • Department of Chemistry, University of California, CA (2015)
  • Department of Biology, Johns Hopkins University, MD (2015)
  • Department of Chemistry and Chemical Biology, Harvard University, MA (2015)
  • European Molecular Biology Laboratory, Heidelberg, Germany (2015)
  • MCDB Graduate Student Symposium, University of Colorado, Boulder, CO (2015)
  • Cold Spring Harbor Asia Conference on Single Cells (2014)
  • Department of Developmental & Stem Cell Biology, Institut Pasteur, Paris, France (2014)
  • Andor Academy, Shanghai, China (2014)
  • Department of Biology, Temple University, Philadelphia, PA (2014)
  • ROEP Annual Symposium, School of Life Sciences, Peking University, China (2014)
  • School of Life Sciences, Tsinghua University, China (2014)
  • Ben May Department of Cancer Research, University of Chicago, IL (2014)
  • Department of Chemistry, Stanford University, CA (2014)
  • Institute for Computational Mathematical Engineering, Stanford University, CA (2014)
  • Biomedical Sciences Program, University of California, Santa Barbara, CA (2014)
  • Pittsburg Conference on Analytical Chemistry and Applied Spectroscopy, Chicago, IL (2014)
  • Biophysical Society 58th Annual Meeting, San Francisco, CA (2014)
  • Department of Physics, Arizona State University, AZ (2014)
  • Workshop on the Advances of Optical Nanoscopy, Centro de Investigaciones en Bionanociencias, Buenos Aires, Argentina (2013)
  • Department of Chemistry, University of Southern California, CA (2013)
  • 10th International Conference on Sampling Theory and Applications (SampTA 2013), Bremen, Germany (2013)
  • Canadian Society for Molecular Biosciences 56th Annual Conference (2013)
  • Department of Molecular and Human Genetics, Baylor College of Medicine, TX (2013)
  • Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, MA (2013)
  • Keystone Symposia on Structural Analysis of Supramolecular Assemblies by Hybrid Methods (2013)
  • Vienna Biocenter, Austria (2012)
  • Computational Optical Sensing and Imaging Graduate Program Seminar, University of Colorado, Boulder, CO (2012)
  • Keystone Symposium on High Throughput Structural Biology, Keystone, CO (2012)
  • EMD Serono Research Institute, MA (2012)
  • Department of Chemistry, Iowa State University, IA (2012)
  • Keystone Symposium on High Throughput Structural Biology, Keystone, CO (2012)
  • New Mexico Center for Spatiotemporal Modeling, Albuquerque, NM (2012)
  • University of Illinois, Urbana-Champaign, Champaign, IL (2011)
  • The Scripps Research Institute, La Jolla, CA (2011)
  • Huazhong University of Science and Technology, Hubei, China (2011)
  • Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun, China (2011)
  • Shanghai Jiaotong University, Shanghai, China (2011)
  • Department of Cellular and Molecular Pharmacology, The University of Tokyo, Tokyo, Japan (2010)
  • The 12th Japanese-American Frontiers of Science Symposium, Chiba, Japan (2010)
  • The 10th International Conference on X-Ray Microscopy, Chicago, IL (2010)
  • The 2nd Workshop on Imaging in Biology and Medicine, Chengdu, China (2010)
  • The First National Conference on Biophysical Chemistry, China, Beijing, China (2010)
  • Stanford University Optical Society, Stanford, CA (2010)
  • Midwest Microscopy and Microanalysis Society, Northwestern University, IL (2010)
  • National Center for Biological Sciences, Bangalore, India (2010)
  • Shenzhen University, Shenzhen, China (2010)
  • Croucher Advanced Study Institute, The Hong Kong University of Science Technology, Hong Kong (2010)
  • 7th NIH Symposium on the Functional Genomics of Critical Illness and Injury, NIH, Bethesda, MD (2009)
  • Department of Chemistry, Hong Kong University of Science and Technology, Hong Kong, (2009)
  • Department of Physics, The Chinese University of Hong Kong, Hong Kong (2009)
  • School of Life Sciences, Tsinghua University, Beijing, China (2009)
  • College of Chemistry and Molecular Engineering, Peking University, Beijing, China (2009)
  • ECI Conference on Advances in Optics for Biotechnology, Medicine and Surgery XI, Burlington, VT (2009)
  • Society of Applied Spectroscopy New England Section March Meeting, Billerica, MA (2008)
  • 19th International Symposium on Microscale Bioseparations, Kobe, Japan (2005)

Professional Activities

  • Instructor, 2010 Bangalore Microscopy Course
  • Instructor, 2011, 2013, 2016 Cold Spring Harbor Laboratory Course in Quantitative Imaging
  • Co-organizer, 2012-2014 QB3-UCSF Course on Biological Microscopy
  • Member, Biophysical Society (2002-Date)
  • Member, American Chemistry Society (2005-Date)
  • Member, American Society of Cell Biology (2008-Date)
  • Journal Reviewer: Cell, Nature, Science, Nature Methods, Nature Biotechnology, Nature Cell Biology, Nature Communications, PNAS, eLife, Developmental Cell, Journal of American Chemical Society, Structure, Nano Letter, Nanoscale, Journal of Molecular Biology, Biophysical Journal, Optica, Optics Letter, Optics Express, Biomedical Optics Express, Microscopy Research and Technique, PLoS One, Scientific Report, etc.