Watching the inner life of a cell
Cellular processes are orchestrated by a large number of protein and nucleic acid molecules in a small volume. In order to elucide how these molecules work together, we take a microscopy approach that systematically maps their spatial organization, activity profile and temporal dynamics.
We are particularly interested in the following problems:
- Physical organization and dynamics of the genome,
- Architecture of large protein complexes such as the centrosome, and
- Subcellular compartmentalization of signaling molecules, particularly
in the G-protein coupled receptors signaling pathway, and how this spatial distribution defines signaling specificity.
In order to study these systems, we are developing the following microscopy technologies:
- Super-resolution and light-sheet microscopes that can visualize subcellular structures at a higher spatial resolution, record long term cell behavior, and track cells in intact animals, and
- New fluorescent probes based on fluorescent proteins, nanobodies and aptamers that reports the localization, conformation and functional modification of proteins and nucleic acids.
|Creating a GFP knock-in library (PNAS 2016)||Protein labeling using FP11 tags (Nat. Comm. 2016)||Multi-color CRISPR imaging (Nucleic Acids Res. 2016)|