Watching the inner life of a cell
Cellular processes are carried out by coordinated participation of many biomolecules in a tiny volume. Many people have been dreaming to see clear pictures of these processes in order to understand how these molecules work together. Taking on this challenge, we are developing new visualization techniques and imaging probes by combining super-resolution microscopy, protein engineering and microfluidic automation. We are particularly interested in studying the following problems:
(1) the protein organization of large macromolecular complexes such as the centrosome,
(2) the physical architecture and dynamics of the genome in the nucleus, and
(3) the spatial distribution of cell surface signaling molecules, particularly G-protein coupled receptors and adhesion molecules, and how this distribution defines their signaling specificity.