Software Plasmids

CRISPR imaging

B. Chen, L.A. Gilbert, B.A. Cimini, J. Schnitzbauer, W. Zhang, G.W. Li, J. Park. E.H. Blackburn, J.S. Weissman, L.S. Qi, B. Huang, "Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System", Cell, 155, 1479-1491(2013) [link]

Link to the Huang lab page on AddGene.

Also see our CRISPR imaging resource page and CRISPR imaging protocols.

Note that AddGene is unable to deliver dCas9-GFP in the Tet-On 3G system due to licensing issues. Therefore, we have deposited a template plasmid for cloning into inducible expressions that can be obtained from commercial or academic channels.